Fig. 1
Effects of M2-type BV-2 conditioned medium and the exosome inhibitor GW4869 on OGD/R-induced ferroptosis in HT-22 cells. (A–C) BV-2 cells were treated with 20 ng/ml IL-4 for 48 h, and the expression levels of CD206 and Arg1 were detected by RT-qPCR, western blot, and immunofluorescence, respectively. Scar bar = 100 μm. (D–H) HT-22 cells were cultured under normal conditions (control), or underwent OGD/R treatment (OGD), or pretreated with M2-BV2-conditioned medium (CM) and exposed to OGD/R treatment (OGD + CM), or pretreated with CM plus GW4869 and exposed to OGD/R treatment (OGD + CM + GW4869). The viability of HT-22 cells in each group was detected by CCK-8 assay (D), the MDA, GSH and iron levels in each group were detected by specific kit, respectively (E–G), and the expression levels of SLC7A11, GPX4 and NCOA4 in each group were detected by western blot (H). **p < 0.01 compared with control group; ##p < 0.01 compared with OGD group; &&p < 0.01 compared with OGD + CM group.
