Fig. 2

Identification of exosomes derived from M2-type BV2 cells (M2-exos). (A) Flow chart of acquisition of exosomes from M2-type BV2 cells. (B) Exosomes were observed under a transmission electron microscope. Scale bar: 200 nm. (C) The size of exosomes was assessed by nanoparticle tracking analysis (NTA). (D) Expression levels of exosomal markers (TSG101, and CD63) and cell-specific markers (GM130 and Calnexin) were measured by western blotting.