Fig. 3
Effect of M2-exos on OGD/R-induced ferroptosis of HT-22 cells. (A) HT-22 cells were cultured under three different conditions: normal (control), OGD/R (OGD), and OGD/R with different concentrations of M2-exos. After 24 h of culture, cell viability was determined by the CCK-8 assay. (B) MSC-exos are internalized by HT-22 cells. PKH26-labeled M2-exos are shown in red, MAP2-labeled HT-22 cells are shown in green, and DAPI-labeled nuclei are shown in blue. Scale bar: 50 µm. (C–F) HT-22 cells were cultured under three conditions: normal (control), OGD/R (OGD), and OGD/R + 40 µg/mL MSC-exos (M2-exos). After 24 h of culture, the MDA, GSH and iron levels in each group were detected by specific kit, respectively (C–E), and the expression levels of SLC7A11, GPX4 and NCOA4 in each group were detected by western blot (F). **p < 0.01 compared with control group; #p < 0.05, ##p < 0.01 compared with OGD group; &p < 0.05, &&p < 0.01 compared with OGD + M2-exos (10 µg/mL) group; @p < 0.05 compared with OGD + M2-exos (20 µg/mL) group.
