Fig. 2 | Scientific Reports

Fig. 2

From: Inhibiting NHEJ in HNSCC cell lines by the ligase IV inhibitor SCR130 has limited radiosensitizing effects

Fig. 2

Flow cytometry analysis of cell death. (A) Gating strategy: Exemplary flow cytometry results of UM-SCC-47 treated with vehicle control (dimethyl sulfoxide, DMSO) or combined treatment (30 µM SCR130 + 2 Gy). First, doublets (I) and cell debris (II) were excluded. Afterwards, the amount of living cells (Annexin7AAD--), apoptotic cells (Annexin7AAD+-), and necrotic cells (Annexin7AAD++) was determined (III). (B) Cell death analysis: apoptotic (Annexin, green), necrotic (7AAD, purple) and sum of dead cells (apoptotic + necrotic, black) in different human papilloma virus (HPV) -negative and HPV-positive HNSCC cell lines and healthy fibroblasts after treatment. Cells were treated with 15 or 30 µM SCR130; half of the samples additionally received 2 Gy IR 3 h afterwards. Vehicle control was treated with the same volume DMSO as used for 30 µM SCR130 (0 Gy). Statistically significant differences were calculated between 2 Gy and 30 µM SCR130 + 2 Gy (sum of dead cells) by two-tailed Mann-Whitney test to evaluate the radiosensitizing effect of SCR130. Each value represents mean ± SD (n ≥ 3).

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