Fig. 6

TIMM8A knockdown promoted apoptosis of breast cancer cells. (A–C) Proportion of apoptotic TIMM8A-knockdown MCF7 and MDA-MB-231 cells generated by flow cytometry using Annexin V-FITC/PI staining. (D–N) The expression levels of apoptotic-related proteins, including BIK, p-BIK, Bcl-2, Caspase-3, and Caspase-9, in MCF7 and MDA-MB-231 cells after knockdown of TIMM8A. GAPDH was used as a loading control. p-BIK levels were normalized with reference to BIK levels. Cleaved-caspase3/9 levels were normalized with reference to pro-caspase3/9 levels. The results shown were representative of at least three independent experiments. The student’s t-test or one-way ANOVA was used to detect the differences among groups. *P < 0.05, **P < 0.01, ***P < 0.001. Samples were derived from the same experiment and gels/blots were processed in parallel. Original blots/gels are presented in the Supplementary File.