Fig. 7
TIMM8A knockdown inhibited the migration and invasion of breast cancer in vitro. (A–C) Migration capacity of MCF7 and MDA-MB-231 cells after knockdown of TIMM8A assayed by scratch experiment. Area Recovery (%) = (initial scratch area—final scratch area)/initial scratch area × 100%. (D–F) Migration capacity of MCF7 and MDA-MB-231 cells after knockdown of TIMM8A assayed by transwell migration assay. (G–I) Invasion capacity of MCF7 and MDA-MB-231 cells after knockdown of TIMM8A assayed by transwell invasion assay. (J–R) The expression levels of NF-κB p65 and EMT-related proteins, including α-SMA, Vimentin, E-cadherin and N-cadherin in MCF7 and MDA-MB-231 cells after knockdown of TIMM8A. GAPDH was used as a loading control. The results shown are representative of at least three independent experiments. EMT, epithelial-mesenchymal transition. The student’s t-test or one-way ANOVA was used to detect the differences among groups. *P < 0.05, **P < 0.01, ***P < 0.001. Samples were derived from the same experiment and gels/blots were processed in parallel. Original blots/gels are presented in the Supplementary File.
