Fig. 4
Psilocybin enhances CB1R expression across four regions in the TRD model compared with WKY rats without substantially diminishing 2-AG levels. (A) 2-AG levels in HYP were quantified using the Luminex assay. 2-AG levels in the SIS subgroup were significantly lower than those in the CTL subgroup. 2-AG levels did not differ significantly within the SIS subgroups. The sample sizes were CTL, n = 5; SIS-Sham, n = 7; and SIS-PSI, n = 7. The data are presented as the mean ± SEM (**p < 0.01) and were analyzed by one-way ANOVA followed by Tukey’s post hoc test. (B) As measured by ELISA, 2-AG levels did not significantly differ in the HIP between the SIS-Sham and SIS-PSI subgroups. (C) Representative WB images illustrating the expression of CB1R in the PFC, AMG, HIP, and HYP of TRD rats exposed to SIS, with GAPDH serving as a loading control. (D) Quantification of CB1Rs in all four brain regions: In the PFC, HIP, and HYP, the CB1Rs were significantly elevated in the SIS-PSI rats compared to those in the SIS-Sham rats. In the AMG, a marginal but nonsignificant increase in CB1Rs was observed in the SIS-PSI rats compared to the SIS-Sham rats. The sample sizes for the SIS-Sham and SIS-PSI groups (B, D) were 5 for each group. The data are presented as the mean ± SEM († p < 0.05, †† p < 0.01) and were analyzed using a two-tailed Mann-Whitney U test for (B) and multiple unpaired t-tests for (D). Data Availability Statement. The datasets generated during and/or analyzed during the current study are available from the corresponding author upon reasonable request.
