Fig. 1

Schematic workflow: Presented in the workflow are the different steps involved in the RSV and HuNoV capture and sequencing methodology. First row—RNA was isolated from mid-turbinate nasal swab samples (RSV) and from stool samples or infected human intestinal enteroids (HuNoV) followed by Real-Time RT-PCR to detect these viruses. Positive samples were quantified, and RNA was converted to cDNA. Second row–The cDNA was used to generate Illumina libraries with molecular barcodes and these libraries were pooled based on the Ct. values. Capture enrichment was performed with either RSV or HuNoV probe set, and enriched libraries were then sequenced on the Illumina NovaSeq 6000 instrument to generate 2 × 150 bp length reads. Pre-captured libraries were also sequenced followed by downstream genome reconstruction, variant, and lineage analyses.