Table 1 Sample composition, mapping, and genome assembly statistics.
Sample details | RSV (pre-capture) | RSV (capture) | HuNov (pre-capture) | HuNov (capture) |
|---|---|---|---|---|
Number of samples | 24 | 85 | 55 | 55 |
Subtype & genotype distribution | RSV-A: 13; RSV-B: 11 | RSV-A: 46; RSV-B: 39 | GI.1: 28; GII.4: 17; Other GII: 10 | GI.1: 29; GII.4: 16; Other GII: 10 |
CT value range | 17.0—29.9 | 17.0—29.9 | 20.2—34.8; ND | 20.2—34.8; ND |
Mapping and assembly statistics | ||||
Raw read count* | 13,387,243 (5,497,877) | 20,583,294 (54,079,506) | 46,289,134 (57,813,028) | 23,991,234 (55,562,802) |
Reads mapping to human* | 6,701,050 (4,587,648) | 300,517 (6,700) | 12,591,275 (40,509,574) | 81,123 (304,768) |
Reads mapping to target virus* | 661 (1,367) | 14,103,914 (39,111,126) | 128,035 (638,298) | 13,644,596 (44,125,310) |
Average genome coverage* | 6 (11) | 123,524 (342,306) | 2,285 (11,398) | 241,333 (782,026) |
Genome length** | 11,984 / 15,253 | 15,116 / 15,346 | 0 / 7651 | 0 / 7,671 |
Genome completeness*** | ||||
Complete genome (correct length range, > 90% complete & > 20x coverage) | 1 (4%) | 85 (100%) | 18 (33%) | 47 (85%) |
Low coverage complete genome (correct length range, > 90% complete, but < 20x coverage) | 6 (25%) | 0 (0%) | 7 (13%) | 2 (4%) |
Incomplete genome (below length range, < 90% complete & < 20x coverage) | 17 (71%) | 0 (0%) | 17 (30%) | 4 (7%) |
No genome assembled | 0 (0%) | 0 (0%) | 13 (24%) | 2 (4%) |
