Fig. 2

Gastrodin treatment reduces markers of ferroptosis in HT22 cells subjected to oxygen-glucose deprivation/reoxygenation (OGD/R). (A) Fe2+ levels; (B) MDA levels; (C) RT-qPCR analysis of SLC7A11 expression; (D–E) Western blot analysis of SLC7A11 protein; (F–H) Western blot analysis of ACSL4 and GPX4 proteins. All data are presented as mean ± SD (n = 3 per group). **P < 0.01, ***P < 0.001 indicate statistical significance.