Fig. 4 | Scientific Reports

Fig. 4

From: Gastrodia protects HT22 cells from damage caused by oxygen glucose deprivation and reperfusion through inhibiting ferroptosis

Fig. 4

Gastrodin inhibited ferroptosis in HT22 cells induced by Erastin. (A) CCK-8 assay of HT22 cell viability; (B) LDH levels in HT22 cells; (C) TUNEL staining of HT22 cell apoptosis rate; (D) Fe2+ levels; (E) MDA levels; (F) RT-qPCR analysis of SLC7A11 expression; (G-H) Western blot analysis of SLC7A11 protein; (I-K) Western blot analysis of ACSL4 and GPX4 proteins. All data are presented as mean ± SD (n = 3 per group). ***P < 0.001 indicates statistical significance.

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