Table 1 Studies of pre-analytical parameters.

From: Evaluation of automatic cell free DNA extraction metrics using different blood collection tubes

Study

Tube type

Multiple time points

Automated extraction

Fluorometric quantification

Single-locus qPCR

Multi-locus qPCR

Electrophoresis

Other assessments

Alidousty et al.52

S,P,R

Yes

Yes

No

Other

–/–

No

No

Barták et al.53

E,S

Yes

Yes

Yes

–/–

–/–

No

Yes

Deleu et al.54

E,Ci

No

Yes

No

–/–

–/–

Yes

Yes

Lampignano et al.55

S,P

No

Yes

Yes

S/L

S/L

Yes

Yes

Lehle et al.56

S

No

Yes

Yes

–/–

S/L

Yes

Yes

Maass et al.37

E,S,P,N

Yes

No

Yes

–/–

–/–

Yes

Yes

Markus et al.57

E,S

Yes

Yes

Yes

–/–

–/–

Yes

Yes

Mehrotra et al.58

E,S

Yes

No

Yes

–/–

S/L

Yes

Yes

Nikolaev et al.59

E,S,P,R

Yes

No

Yes

S/L

–/–

Yes

No

Parackal et al.60

E,S,R

Yes

No

Yes

–/–

–/–

Yes

No

Pedini et al.61

R

N/A

Yes

Yes

–/–

–/–

Yes

Yes

Pérez-Barrios et al.62

E

No

Yes

Yes

–/–

–/–

Yes

Yes

Polatoglou et al.63

E

No

Yes

Yes

–/–

–/–

Yes

No

Risberg et al.64

E,S

Yes

No

No

–/–

–/–

No

Yes

Samoila et al.65

E,S,P

Yes

Yes

No

–/–

–/–

Yes

Yes

Schmidt et al.66

E,P

Yes

Yes

No

S/L

–/–

No

Yes

Stasik et al.67

E,S,P

Yes

Yes

No

S/–

–/–

No

Yes

Streleckiene et al.68

E

No

No

Yes

–/–

–/–

Yes

No

Streubel et al.69

E,P

No

Yes

Yes

S/–

–/–

Yes

Yes

Terp et al.48

E

No

Yes

No

–/–

–/–

Yes

Yes

van Dessel et al.70

E,S,Cs

Yes

No

Yes

–/–

–/–

No

Yes

van Dessel et al.46

E,Cs

Yes

Yes

Yes

S/–

–/–

No

Yes

van Ginkel et al.71

E,S,Cs,Ci,H

Yes

Yes

Yes

–/–

–/–

No

Yes

Ward Gahlawat et al.47

E,S,P,N,R

Yes

No

Yes

–/–

–/–

Yes

Yes

Warton et al.72

E,S,P

Yes

No

No

S/–

S/L

Yes

No

Wolf et al.73

E,S

Yes

Yes

Yes

–/–

S/–

Yes

Yes

Zhao et al.74

E,S,R

Yes

No

No

–/–

S/–

No

Yes

  1. Tube type: S, Streck; P, PAXgene; R, Roche Cell-Free DNA Collection Tube; E, EDTA; Ci, Citrate; N, Norgen; Cs, CellSave preservative tubes; H, Heparin. Multiple time points: different times between sample collection and plasma isolation were tested. Single-locus qPCR assessing short (S, < 150 bp) and long (L, > 200 bp) target sequences. Other: assay of unknown target(s) and size. Multi-locus qPCR assessing short (S, < 150 bp) and long (L, > 200 bp) target sequences.
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