Table 1 Contrast values (Group 1 – Group 2) for comparisons among periods within sites and sites within period for both winter experiments (2023-24 and 2024-25).

From: Temperature and CO2 concentration in honey bee hives exhibit circadian rhythms

Parameter

Group 1

Group 2

Contrast value

Watson

P

Site

Interval

Site

Interval

Temperature

CSU

7–12 d

CSU

25–30 d

5.920

0.044

> 0.1

CSU

7–12 d

CSU

40–45 d

0.514

0.120

> 0.1

CSU

7–12 d

out

post

3.032

0.479

< 0.001

CSU

25–30 d

CSU

40–45 d

0.877

0.073

> 0.1

CSU

25–30 d

out

post

3.395

0.632

< 0.001

CSU

40–45 d

out

post

2.518

0.880

< 0.001

CO2

CSU

7–12 d

CSU

25–30 d

3.409

0.372

< 0.01

CSU

7–12 d

CSU

40–45 d

4.157

0.374

< 0.01

CSU

7–12 d

out

post

0.997

0.432

< 0.001

CSU

25–30 d

CSU

40–45 d

0.748

0.097

> 0.1

CSU

25–30 d

out

post

3.871

0.521

< 0.001

CSU

40–45 d

out

post

3.123

0.760

< 0.001

  1. “Site” indicates whether the data are from colonies within the CSU (“CSU”) or outside in ambient conditions (“out”). “Interval” indicates the 6-d interval, with each interval consisting of two sequential 3-d subsets; during interval “7–12 d” colonies were in total darkness; during intervals “25–30 d” and “40–45 d” colonies were subject to a 12 l:12D light regime; and during interval “post” colonies were in ambient conditions outside the CSU. “Watson” indicates the value of the Watson’s test for homogeneity on two samples of circular data, and “P” refers to the probability of the value. N = 32 for each group within the CSU; N = 30 for the post-CSU temperature group and N = 26 for the post-CSU CO2 group (see text for details). Significant contrasts are in bold.