Fig. 2
From: The role of HMOX1-mediated ferroptosis in blue light-induced damage to retinal pigment epithelium
Ferrous ion-catalysed oxidation reaction was involved in blue light-induced ferroptosis in ARPE-19 cells. (A) FerroOrange staining and (B) the relative fluorescence intensity of ferrous ions. Scale bar: 50 μm. n = 3, one-way ANOVA with Tukey’s post hoc test, **p < 0.01, data presented as mean ± SD. (C) CM-H2DCFDA staining and (D) the relative quantitation of intracellular ROS levels in ARPE-19 cells. Scale bar: 50 μm. n = 3, one-way ANOVA with Tukey’s post hoc test, **p < 0.01, data presented as mean ± SD. (E) BODIPY staining of LOS generation in ARPE-19 cells and (F) ratio of oxidative BODIPY. Scale bar: 50 μm. n = 3, one-way ANOVA with Tukey’s post hoc test, **p < 0.01, data presented as mean ± SD.
