Fig. 3

HMOX1 upregulation contributed to ferroptosis in ARPE-19 cells. (A) The number of differentially expressed genes (upregulated in red and downregulated in green) was presented in a volcano plot from RNA-seq analysis. (B) Differentially expressed genes closely associated with ferroptosis in blue light-treated ARPE-19 cells were exhibited in heatmap. (C) Western-blot assay, and (D) relative protein quantitation of HMOX1 and SLC7A11 after treatment with blue light. n = 3, two-tailed Student’s t-test, **p < 0.01, data presented as mean ± SD. Original blots are presented in Supplementary Fig. 6. (E) Western-blot assay, and (F) relative protein quantitation of the upstream transcription factors, AP-1, NF-κB, and Nrf2. n = 3, two-tailed Student’s t-test, **p < 0.01, not significant (N.S.) > 0.05, data presented as mean ± SD. Original blots are presented in Supplementary Fig. 7.