Fig. 5
From: The role of HMOX1-mediated ferroptosis in blue light-induced damage to retinal pigment epithelium
Inhibition of HMOX1 upregulation prevented retinal damage caused by blue light exposure in vivo. (A) H&E staining showed pigmentation and morphological changes in the RPE pretreated with the HMOX1 inhibitor ZnPP, followed by blue light treatment. Scale bar: 25 μm. Black arrows: RPE cells. ONL: outer nuclear layer, INL: inner nuclear layer. (B) Immunofluorescent staining and (C) relative fluorescence intensity of RPE tight junction biomarker ZO-1. Scale bar: 25 μm. n = 5, one-way ANOVA with Tukey’s post hoc test, **p < 0.01, data presented as mean ± SD. (D) The visual functions of mice in the control, blue light and ZnPP groups were detected through ERG. (E) ERG a- and b-wave amplitude values were measured. n = 5, one-way ANOVA with Tukey’s post hoc test, **p < 0.01, data presented as mean ± SD. (F) After mice with different treatment for 14 days, immunofluorescence staining of typical photoreceptor biomarkers, Arrestin in mouse retina was performed. Scale bar: 100 μm. Hoechst (blue): nuclei. Arrestin: red. ONL: outer nuclear layer, INL: inner nuclear layer, GCL: ganglion cell layer.
