Fig. 1 | Scientific Reports

Fig. 1

From: Decreased expression of insulin-degrading enzyme increases gluconeogenesis and glucose production in cultured hepatocytes administered with glucagon

Fig. 1

Effects of liver-specific depletion of IDE on the glucagon signaling pathway. Livers from fasted 3-month-old WT and L-IDE-KO mice were excised and protein lysates were prepared by homogenizing the tissues in lyses buffer. (A) Representative western blot of liver lysates (40 µg protein/sample) from WT (white bars) or L-IDEKO (black bars). (B) Densitometric analyses of the data in panel A of the glucagon receptor (GCGR), (C) p-CREB, (D) CREB and (E) the ratio p-CREB versus total CREB protein. Data are expressed relative to WT. Mean ± SEM for n = 4 independent experiments per genotype. *p value < 0.05 versus WT by Students´ T-test.

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