Fig. 2 | Scientific Reports

Fig. 2

From: Decreased expression of insulin-degrading enzyme increases gluconeogenesis and glucose production in cultured hepatocytes administered with glucagon

Fig. 2

Effects of genetic depletion of IDE on glucagon signaling and expression of gluconeogenic genes in primary mouse hepatocytes. Primary hepatocytes isolated from fasted 3-month-old WT and L-IDE-KO mice were treated with glucagon (50 ng/mL) at the indicated times followed by quantification of protein levels of the glucagon signaling pathway. (A) Representative western blot (40 µg protein/sample) depicting WT (white bars) and L-IDE-KO (black bars) mouse primary hepatocytes treated with glucagon. Densitometric analyses of the data in panel A of the GCGR (B), p-CREB (C), CREB (D) and the ratio p-CREB versus total CREB protein (E). Data are expressed relative to WT. Mean ± SEM for n = 3 independent experiments per genotype. *p value < 0.05 versus WT by two-way ANOVA. #p value < 0.05 versus untreated cells (time 0) by two-way ANOVA. Primary hepatocytes isolated from WT and L-IDE-KO mice were treated with glucagon as above followed extraction and quantification of mRNA levels of Pck1 (F) and G6pc (G). Data are mean ± SEM (relative to control) for n = 3–6 independent experiments in triplicate per genotype and condition. *p value < 0.05 versus WT by Students’ T-test.

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