Fig. 8

STRING analysis of differentially represented proteins present in L. reuteri DSM 17938 pMVs and bMVs. Panel (A). Protein interaction network of differentially represented proteins (87 in number) identified in freshly prepared L. reuteri pMVs and bMVs. Functional protein associations were based on the corresponding data recorded in the STRING database. Medium-confidence interactions (0.4) are shown. Proteins involved in microbial metabolism in diverse environments and bacterial signaling are shown in blue and red, respectively. ackA, acetate kinase; EDX43163.1, aldo/keto reductase; EDX43448.1, malate dehydrogenase; EDX41751.1, ROK family protein; EDX42770.1, hypothetical protein LOC688242; prsA, ppic-type peptidyl-prolyl cis-trans isomerase; EDX43142.1, 6-phosphogluconate dehydrogenase, decarboxylating; EDX41882.1, putative aspartate aminotransferase; eno, phosphopyruvate hydratase; EDX41874.1, Udp-glucose 4-epimerase; EDX41675.1, histone-like DNA-binding protein wrapping DNA to stabilize it; EDX43505.1, substrate-binding region of ABC-type glycine betaine transport system; EDX42754.1, mannosyl-glycoprotein endo-β-N-acetylglucosamidase; rplJ, ribosomal protein L10; EDX41915.1, phosphoglucomutase/phosphomannomutase alpha/beta/alpha domain I; EDX43238.1, alcohol dehydrogenase GroES domain protein; EDX41957.1, acyltransferase 3; EDX43169.1, cysteine synthase/cystathionine beta-synthase family protein; EDX42851.1, mannosyl-glycoprotein endo-beta-N-acetylglucosamidase; EDX41492.1, membrane-associated zinc metallopeptidase M50; EDX41826.1, polar amino acid ABC transporter, inner membrane subunit; EDX43399.1, beta-phosphoglucomutase; EDX41844.1, putative transcriptional regulator, GntR family; EDX42772.1, peptidase M23; EDX42605.1, histidine triad (HIT) protein; tsf, translation elongation factor ts; EDX42550.1, penicillin-binding protein transpeptidase; EDX42636.1, dipeptidase M20; groS, chaperonin cpn10; EDX43274.1, protein of unknown function DUF1002; EDX41827.1, ABC-type polar amino acid transport system, ATPase component; EDX43144.1, PTS system, glucose subfamily, IIA subunit; EDX43264.1, peptidase M1 membrane alanine aminopeptidase; metK, methionine adenosyltransferase; EDX43570.1, conserved hypothetical protein; pgi, glucose-6-phosphate isomerase; EDX43174.1, peptidase M13 neprilysin; EDX42202.1, conserved hypothetical protein; EDX42038.1, conserved hypothetical protein; EDX42068.1, conserved hypothetical protein; EDX42901.1, alcohol dehydrogenase GroES domain protein; EDX43434.1, inosine/uridine-preferring nucleoside hydrolase; EDX42541.1, glutamine synthase; apt, adenine phosphoribosyltransferase; rbsK, ribokinase; EDX43429.1, ABC-type amino acid transport system, permease and periplasmic component - ionotropic glutamate receptor; EDX43433.1, sodium-dependent nucleoside transporter; EDX42434.1, ribulose-phosphate 3-epimerase family protein; EDX42771.1, hypothetical protein; EDX41825.1, cystathionine gamma-synthase; tuf, translation elongation factor tu; EDX42032.1, universal stress protein; ddl, D-alanine/D-alanine ligase; EDX42537.1, nlpA lipoprotein family protein; EDX41856.1, iron-containing alcohol dehydrogenase family protein; EDX43442.1, membrane protein of unknown function UCP033111; EDX41992.1, conserved hypothetical secreted protein; EDX41978.1, ornithine carbamoyltransferase; EDX41828.1, bacterial solute-binding protein 3 family protein; EDX43074.1, ferritin dps family protein; EDX41701.1, protein of unknown function DUF322; EDX43359.1, peptidase S1 and S6 chymotrypsin/Hap; EDX43514.1, nlpA lipoprotein family protein; EDX41943.1, phosphate acetyltransferase; rpsE, ribosomal protein S5; groL, chaperonin GroEL; EDX43230.1, peptidoglycan-binding LysM; EDX42138.1, cell division initiation protein; EDX43365.1, 1,3-propanediol dehydrogenase; EDX41765.1, penicillin-binding protein, 1 A family; EDX42825.1, redoxin domain protein; pgk, phosphoglycerate kinase family protein; EDX41762.1, conserved hypothetical protein; EDX42769.1, hypothetical protein; EDX41555.1, pyruvate kinase family protein; EDX42065.1, glutamine ABC transporter substrate binding component; ldh, L-lactate dehydrogenase. Panel (B). Functional analysis of differently represented proteins identified in freshly prepared L. reuteri pMVs and bMVs. Differentially represented proteins (87 in number) were subjected to functional enrichment based on KEGG pathway database. The top-10 pathways are reported. Shown is the pathway code, the corresponding description, count in network, strength and false discovery rate (FDR).