Fig. 6
Anti-inflammatory properties of BmKTX-33. (a,b) CLSM images of RAW264.7 cell surface-specific markers CD86 and CD206. Green represented M1 type macrophages; red represented M2 type macrophages. (c,d) The proportion of M1- or M2-type macrophages. (e–g) The secretion of IL-1β (e), NO (f) and IL-10 (g) in the supernatants of different treatment groups as detected by ELISA. BmKTX-33–1 represented the concentration of BmKTX-33 (62.5 μg/mL); BmKTX-33–2 represented the concentration of BmKTX-33 (125 μg/mL); BmKTX-33–3 represented the concentration of BmKTX-33 (250 μg/mL). Data were generated from at least three independent experiments and presented as mean ± standard deviation. (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).
