Fig. 4
From: Characterization of odor markers associated with aging in old ICR mice
Transcription level of the gene encoding TMA metabolic enzyme. (A) Biochemical reaction of TMA. This compound was catalyzed into TMAO through TMA monooxygenase (FMO3). (B) Transcript levels of gene encoding FMO3. After purifying the total mRNA of the liver and foot tissue, transcription levels of this gene were detected by qRT-PCR analysis using specific primers. The preparation of the total RNAs from the liver and feet tissues was performed on three to five mice per group, and the levels of the PCR product were analyzed twice for each sample. The data represents the means ± SD. ∗p < 0.05 compared with the 2-months-old group. #p < 0.05 compared with the 6-month-old group. Abbreviations: TMA: Trimethylamine, TMAO: Trimethylamine N-oxide, FMO3: Flavin-containing monooxygenase 3.
