Fig. 1
From: Crosstalk of injured podocytes with parietal epithelial cells through Wnt4/β-Catenin signaling
DT-induced podocyte loss increases proteinuria, FSGS lesions and PEC migration to the glomerular tuft. Proteinuria was assessed for rats using metabolic cages; DT injected rats grouped by sex vs. PBS injected rats (●male/▲female vs. ■controls) (A). Representative pictures of the WT1-staining in healthy control (CTL) and DT-treated rats on day 28 are shown (B). Depletion of WT1-positive cells per glomerular cross section (gcs) in DT-injected rats compared to controls over time (C). The percentage of FSGS-lesions in male and female rats, as assessed on PAS-stained sections, is shown for all time points (D). The number of Pax8-positive cells on the glomerular tuft per glomerular cross section was evaluated using immunohistochemistry (E). The percentage of FSGS-lesions correlate with Pax8-positive cells per gcs (F). Representative images of a glomerular section with FSGS lesion in a rat 35 days after DT injection stained by immunohistology for Pax8 (G, arrows) and the subsequent section with synaptopodin (H) with a dashed line to mark the FSGS lesion. *p < 0.05 **p < 0.01 ***p < 0.001; colored asterisks indicate significance within each sex.
