Fig. 4
From: Crosstalk of injured podocytes with parietal epithelial cells through Wnt4/β-Catenin signaling
Colocalization of Wnt4 and β-Catenin in podocytes and PECs after induction podocyte injury. Immunofluorescence triple staining marking Wnt4 (A, B) or β-Catenin (C-E) in red, nephrin in green und Pax8 positive nuclei in blue. Colocalization of Wnt4 with nephrin and Pax8 was demonstrated in healthy rats (A) and on day 14 after DT-injection (B). Colocalization of β-Catenin with nephrin and Pax8 was demonstrated in healthy rats (C) and on day 14 (D) and on day 28 after DT-injection (E). In picture (B) arrowheads mark Pax8-positive PECs on the glomerular tuft, while in figure (D) and (E) arrowheads mark intense staining for β-Catenin in FSGS lesions. Scale bars represent 100 µm.
