Fig. 6
From: Crosstalk of injured podocytes with parietal epithelial cells through Wnt4/β-Catenin signaling
Wnt4 is secreted by injured podocytes that can elicit Wnt4 downstream targets in PECs. A schematic overview of the experimental setup of the in vitro experiments is shown (for details see M&M section) (A). mRNA expression of Wnt4 (B), axin2 (C) and β-Catenin (D) was assessed in cultured podocytes stimulated for 24 and 48 h with 30 µg puromycin amino nucleoside (PAN) compared to untreated controls (CTL). mRNA expression of Axin2 (E) and β-Catenin (F) was analyzed in mouse PECs incubated with PEC medium (CTL), podocyte medium (FCS), supernatant from PAN-stimulated podocytes (SN) or 50 ng/ml recombinant Wnt4 (Wnt4) with ( +) and without (unmarked) the addition of the Wnt inhibitor dickkopf-related protein 1 (DKK-1) using Real-time PCR. *p < 0.05 **p < 0.01 ***p < 0.001.
