Fig. 7
From: Crosstalk of injured podocytes with parietal epithelial cells through Wnt4/β-Catenin signaling
Wnt4 is expressed in glomeruli of human biopsies with primary FSGS. The percentage of glomerular cross-sections (gcs) with Pax8-positve cells on the glomerular tuft (A) and the numbers of Pax8-positive cells in gcs with Pax8-positive cells (B) were analyzed in human kidney biopsies from patients with acute tubular injury or hereditary collagen IV-associated disease (CTL, n = 19), minimal change glomerulonephritis (MCD, n = 22) and podocytopathic focal segmental glomerulosclerosis (FSGS, n = 19). Pax8-positive cells on glomerular tuft correlated positively with the percentage of glomeruli with FSGS lesion (C). Glomerular Wnt4-staining by immunohistochemistry was analyzed using a semi-quantitative score (D). In human kidney biopsies from patients with primary FSGS CD44 (brown staining) colocalized with Pax8 (grey staining) in glomerular cells using immuno-double staining (E). Double staining of CD44 (red staining) with nephrin (green staining) showed that CD44 was not expressed by nephrin-positive podocytes (F). *p < 0.05 **p < 0.01 ***p < 0.001; colored asterisks indicate significance within each sex.
