Fig. 3 | Scientific Reports

Fig. 3

From: Endoplasmic reticulum stress sensor protein PERK in hepatic stellate cells promotes the progression of hepatocellular carcinoma via p38δ MAPK/IL-1β axis

Fig. 3

PERK overexpression increases IL-1β expression in HSCs. PERK is overexpressed in LX-2 cells using a plasmid, and the expression of PERK, downstream molecules of PERK, and IL-1β is examined. (a) PERK mRNA expression is evaluated using RT-PCR (n = 4). (b) The expression of GFP-PERK, PERK, p-eIF2α, and eIF2α is determined using WB. Supplementary Fig. S6 presents the original membranes of WB. (c) IL-1β mRNA expression is evaluated by RT-PCR (n = 4). (d) IL-1β protein expression is determined using WB. Supplementary Fig. S7 presents the original membranes of WB. Data are shown as the mean ± SE. Statistical analysis is performed using Student’s t-test. **p < 0.01. Abbreviations: PERK, Protein kinase R-like endoplasmic reticulum kinase; IL-1β, Interleukin-1 beta; GAPDH, Glyceraldehyde 3-phosphate dehydrogenase; WB, Western blotting; RT, Reverse transcription; siRNA, Small interfering RNA; HSCs, Hepatic stellate cells; SE, Standard error; GFP, Green fluorescent protein; p-eIF2α, Phosphorylated eukaryotic initiation factor 2-alpha.

Back to article page