Fig. 7

p38δ MAPK regulates IL-1β expression in HSCs. p38δ MAPK knockdown is performed in LX-2 cells using siRNA, and the expression of p38δ MAPK and IL-1β is verified. (a) The mRNA expression of p38δ MAPK and IL-1β are evaluated using RT-PCR (n = 4). (b) p38δ MAPK and IL-1β protein expression are determined by WB. Supplementary Fig. S10 presents the original membranes of WB. p38δ MAPK is overexpressed in LX-2 cells using a plasmid, and the expression of p38δ MAPK and IL-1β is assessed. (c) p38δ MAPK and IL-1β mRNA expression are evaluated using RT-PCR (n = 4). (d) p38δ MAPK and IL-1β protein expression are determined using WB. Supplementary Fig. S11 presents the original membranes of WB. Data are shown as the mean ± SE. Statistical analysis is performed using Student’s t-test. *p < 0.05, **p < 0.01. Abbreviations: IL-1β, interleukin-1 beta; MAPK, mitogen-activated protein kinase; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; WB, western blotting; RT, reverse transcription; siRNA, small interfering RNA; HSCs, hepatic stellate cells; SE, standard error.