Fig. 3

RNA sequencing analysis of the transcriptomal features in CML cells. (A, B) K562 cells were treated with imatinib (0.5 µM) in the presence or absence of MLN4924 (25 µM) for 12 h, RNA sequencing was performed. PCA analysis (A) and sample correlation analysis (B) were performed. (C) GO analysis of the DEGs between imatinib group and imatinib + MLN4924 group. (D-H) Construction of WGCNA network of all RNA sequencing samples: (D) Dendrogram of gene topological matrix branch. (E) Dendrogram and correlation heatmap of 10 eigengenes in each module. (F) Heatmap of correlations between gene modules and each group of RNA-seq samples. The Pearson correlation coefficient (R) between different gene modules and clinical traits were shown in the heatmap. (G) Scatter plot of gene significance for selected groups with module membership (MM) in the most significantly positively correlated module. The brown module showed significant positive correlations with both MLN and IMA + MLN groups. (H) GO enrichment analysis of genes in blue, red, turquoise, and brown modules.