Fig. 1
From: PTPN2 inhibits TG-induced ERS-initiated TNBC apoptosis through the mitochondrial pathway
PTPN2 directly interacts with and inhibits GRP78 in TNBC. (A) The Western blot shows a direct interaction of PTPN2 45 kDa or PTPN2 48 kDa with GRP78. The protein lysate was precipitated with anti-IgG or anti-Flag, and blot with anti-GRP78 or anti-Flag. Input: whole cell lysate; IgG: negative control; Flag: representing PTPN2 45 kDa or PTPN2 48 kDa. (B) The Western blot shows ectopic expression of PTPN2 45 kDa or PTPN2 48 kDa diminishes GRP78 expression in TG-treated MDA-MB-231 and SUM159 cells, most obviously at 48 h. PTPN2 serves to indicate over-expression of PTPN2, and GAPDH serves as a loading control. All experiments are conducted as a single independent experiment (n = 1). (C) The Western blot shows knockdown of PTPN2 augments GRP78 expression in TG-treated MDA-MB-231 and SUM159 cells, most obviously at 48 h. PTPN2 serves to indicate knockdown of PTPN2, and GAPDH serves as a loading control. All experiments are conducted as a single independent experiment (n = 1).
