Fig. 5
From: PTPN2 inhibits TG-induced ERS-initiated TNBC apoptosis through the mitochondrial pathway
PTPN2 inhibits TG-initiated autophagy in TNBC. (A) The western blot shows that ectopic expression of PTPN2 45 kDa or PTPN2 48 kDa decreases LC3 II/LC3 I ratio and increases P62 expression, indicating PTPN2 inhibits autophagy in TG-treated MDA-MB-231 and SUM159 cells. GAPDH serves as a loading control. All experiments were conducted two times independently (n = 2). (B) The western blot shows that knockdown of PTPN2 increases LC3 II/LC3 I ratio and decreases P62 expression, indicating knockdown of PTPN2 promotes autophagy in TG-treated MDA-MB-231 and SUM159 cells. GAPDH serves as a loading control. All experiments were conducted two times independently (n = 2). (C) The representative fluorescence images of Annexin V/ PI staining demonstrating apoptotic cells shows that knockdown of PTPN2 promotes cell apoptosis in TG-treated MDA-MB-231 cells, and the presence of CQ further elevates cell apoptosis. Scale bar = 200um. All experiments were conducted three times independently (n = 3). (D) The bar graph shows that knockdown of PTPN2 promotes cell apoptosis in TG-treated MDA-MB-231 cells, and the presence of CQ further elevates cell apoptosis by quantifying apoptotic cells in 3 different fields under the microscope. (E) The western blot shows that knockdown of PTPN2 increases expression of cleaved Caspase3 and the ratio of LC3 II/LC3 I. Presence of CQ further elevates expression of cleaved Caspase3 and the ratio of LC3 II/LC3 I, indicating CQ presence promotes apoptosis in TG-treated MDA-MB-231 cells. GAPDH serves as a loading control. All experiments are conducted as a single independent experiment (n = 1).
