Fig. 1
From: Gradual transition of pyramidal cell types in the dorsal hippocampal area CA2b of the C57BL/6 mouse
Identification of PCP4-positive cells in sections of brain slices filled iontophoretically with an Alexa dye. (a) Neurons along the CA3-CA2-CA1 axis of the dorsal hippocampus were iontophoretically injected with a fluorescent Alexa dye (Alexa 568 or Alexa 488) in 250 μm thick fixed brain sections. Sections were subsequently resliced to 40 μm to facilitate the following PCP4 staining. (b) Filled, resliced and stained sections were imaged and maximum-Z-projections were used to define the borders of the CA regions using the PCP4 staining in the section containing the cell somata. Cell bodies of filled cells were then numbered and identified as PCP4-positive or PCP4-negative in higher-magnification images. (c) If proximal apical dendrites were not on the same section as the cell bodies, dendrites on adjacent sections were assigned to their respective cell bodies on the basis of their location within the section. (d) Relative positions of all 90 evaluated filled neurons from a total of five male and six female C57BL/6 mice. SO - stratum oriens; SP - stratum pyramidale; SL - stratum lucidum; SR - stratum radiatum; CA - cornu ammonis.
