Table 1. R7 modulated the expression of several genes as identified by an mRNA microarray, followed by filtering for hits that came up in HPK. HPK from four different donors (1-4) were either not stimulated or stimulated with 10 µg/ml R7. After 6 h, cells were harvested and RNA was isolated. The respective samples from two donors (1+2; 3+4) were pooled and the resulting 4 samples (unstimulated, stimulated with R7, respectively for HPK pooled from donors 1+2 or 3+4) were analyzed using a whole human genome oligo-microarray. For upregulated transcripts, the stimulated/unstimulated ratio is shown. For downregulated transcripts, the unstimulated/stimulated * (-1) ratio is indicated. We filtered for hits that were consistently observed after R7 stimulation in both sets of pooled keratinocytes. In the first column, protein-coding targets are shown in black, RNA genes in blue. Involvement in inflammation, skin integrity or atopy is indicated in red, involvement in splicosome function in green. In columns 2-3, uphits with a stimulation of > 2-fold are shown in orange. Downhits with a> 2-fold reduction are displayed in green. In the fourth column, the same trend with at least two probes is indicated in orange.

From: RNase 7 and Th cytokines synergistically increase the secretion of interleukin-6 from keratinocytes