Table 3 Comparison of different methods for the determination of antinuclear antibodies (ANA) and ANA-specific autoantibodies.
From: CytoBead ANA 2 assay - a novel method for the detection of antinuclear antibodies
CytoBead ANA | Cohen’s κ* (95% CI) | Difference (%) (95% CI) | p** | ||
|---|---|---|---|---|---|
Neg | Pos | ||||
Classical ANA IIF | 0.74 (0.61–0.88) | 9.71 (3.39–16.03) | 0.006 | ||
Neg | 29 | 11 | |||
Pos | 1 | 62 | |||
CytoBead ANA | |||||
|---|---|---|---|---|---|
Neg | Pos | ||||
BioPlex | |||||
Anti-SS-B/La | 0.9 (0.76–1.00) | 1.79 (−0.67–4.24) | 0.500 | ||
Neg | 100 | 2 | |||
Pos | 0 | 10 | |||
Anti-CENP/CENP-B | 0.9 0.72–1.00 | −0.89 (−2.64–0.85) | 1.000 | ||
Neg | 106 | 0 | |||
Pos | 1 | 5 | |||
Anti-Sm | 0.76 0.56–0.96 | −4.46 (−8.29 - −0.64) | 0.062 | ||
Neg | 98 | 0 | |||
Pos | 5 | 9 | |||
Anti-RNP/Sm | 0.7 0.41–0.98 | 1.79 (−1.70–5.27) | 0.625 | ||
Neg | 103 | 3 | |||
Pos | 1 | 5 | |||
Anti-dsDNA | 0.72 0.51–0.93 | 3.57 (−0.66–7.81) | 0.212 | ||
Neg | 97 | 5 | |||
Pos | 1 | 9 | |||
Anti-Scl70 | 0.79 0.51–1.00 | 0 (−2.47–2.47) | 1.000 | ||
Neg | 106 | 1 | |||
Pos | 1 | 4 | |||
Anti-SS-A/Ro | 0.87 0.77–0.96 | 4.46 (−0.09–9.02) | 0.125 | ||
Neg | 66 | 6 | |||
Pos | 1 | 39 | |||
Anti-Jo-1 | 0.88 0.66–1.00 | 0.89 (−0.85–2.64) | 1.000 | ||
Neg | 107 | 1 | |||
Pos | 0 | 4 | |||
