Fig. 2
Expression analysis of NaLRR-RK4 and disease phenotype in tobacco under Alternaria alternata treatment (Mean ± SD). (a) Relative expression levels of NaLRR-RK4 in tobacco leaves at 1 and 3 days post-A. alternata inoculation, based on transcriptome data. (b) RT-qPCR analysis of NaLRR-RK4 expression in WT and RNAi plants under both inoculated and non-inoculated conditions at 1 and 3 days. (c) Lesion diameters in WT and RNAi plants at 3 days after A. alternata inoculation. Here, RNAi_un represents silenced plants without A.alternata inoculation, RNAi_in represents silenced plants with A.alternata inoculation, WT_un represents wild-type plants without A.alternata inoculation, and WT_in represents wild-type plants with A.alternata inoculation. The red asterisks (“**” for P < 0.01 and "***" for P < 0.001) indicate statistically significant differences among treatments, as determined by Tukey’s test.
