Fig. 6 | Scientific Reports

Fig. 6

From: The mechanism of Shancigu and its monomer in the development of colorectal cancer based on network pharmacology

Fig. 6

Stigmasterol regulated CRC cell viability, proliferation, apoptosis, migration, and invasion. A CCK-8 assay detection of HCT116 and Caco-2 cell viability. B Colony formation assay assessed the long-term proliferative capacity of HCT116 and Caco-2 cells following Stigmasterol treatment. C Flow cytometry determination of HCT116 and Caco-2 cell apoptosis. D Transwell assays detection of HCT116 and Caco-2 cell migration and invasion abilities. E Western blot analysis of Stigmasterol-binding proteins ITGB1 and JUN expressions. Data represent the mean ± SD of three independent biological replicates. *P < 0.05, **P < 0001, ***P < 0.001.

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