Fig. 1
From: Volatilomic response to targeted cancer therapy in vitro
(A-B) Dose-response curves of mTORci sensitive (HCT116) and resistant (HT29) cell lines treated with OSI-027 and AZD2014 respectively for 72 h. Percentage cell viability was normalised against DMSO vehicle control. n = 6. (C) Representative western blot demonstrating abolished p-4EBP1 protein expression in HCT117 sensitive cells treated with mTORci at IC50. n = 3. (D) Kaplan-Meier plot showing significantly higher survival in patients with low p-4EBP1 protein expression in TCGA-COAD cohort. (E-G) Gene set enrichment scores of TCGA-COAD dataset stratified by p-4EBP1 protein expression. (F) Representative Images of confocal microscopy (20x) demonstrating autophagy induction (LC3 staining) in mTORci-sensitive cell line post-treatment. Cells were counterstained with DAPI (nuclei) prior to mounting. n = 3.
