Fig. 2
From: Volatilomic response to targeted cancer therapy in vitro
(A) Overview of bespoke workflow used for paired untargeted lipid-VOC phenotyping. (B) Untargeted lipidomics analysis identified the top 8 differential unsaturated phospholipids between cell lines, presented as fold changes (FC) relative to DMSO vehicle control. (C, D) Paired lipid extracts underwent ambient auto-oxidation for 72 h in headspace reactions prior to untargeted GC-MS analysis. Orthogonal partial least squares-discriminant analysis was used to identify VOCs that are associated with mTORci response, comparing controls with drug treatment groups. (E-G) Distinct volatile signatures generated from lipid extracts of mTORci-treated, sensitive and resistant cells. VOCs differentially enriched (VIP > 1) were plotted. Two-way ANOVA was performed with Tukey multiple comparison against DMSO controls. n = 3. **p < 0.01, ***p < 0.001, ****p < 0.0001. Figure 2 A was created in BioRender. Leung, P. (2025) and is accessible through https://BioRender.com/47kxskl on a CC-BY 4.0 license.
