Fig. 6
From: Effects of ATF2/TSC1 on epilepsy by modulating the microphages polarization of microglia
ATF2 regulates TSC1 transcription. (A) The binding sites of ATF2 obtained from JASPAR database. (B) The promoter regions (P1: promoter region 1, P2: promoter region 2, P3: promoter region 3) of TSC1. (C) The relative enrichment of ATF2 in TSC1 promoter regions. Dual luciferase reporter (D) and western blot (E) assay revealed that ATF2 inhibited the transcription of TSC1. (F) The luciferase activity of wild-type (WT) and mutant (MUT) TSC1 in HMC3 cells. (G) Relative expression of TSC1 in KA-induced HMC3 cells. (H-I) Transfection with sh-ATF2 enhanced TSC1 protein. *P < 0.05, **P < 0.01, ***P < 0.001.
