Fig. 1
Effects of WA and IR on cell viability and apoptosis. (A) Cell viability of SGC-7901, HUVEC, and IEC-6 cells treated with varying concentrations of WA (1, 2, 4, 8, 10, 12, 16, 20, 22, or 24 µM) for 30 min, followed by 10 Gy IR, assessed via CCK-8 assay. Viability values were normalized to the untreated control (0 µM WA, no IR). (B-D) Determination of half-maximal inhibitory concentration (IC50) of WA in SGC-7901, HUVEC and IEC-6 cells. Viability values were normalized to the IR-only group (0 µM WA + IR) and used to calculate IC50 values under irradiated conditions. (E) Expression of cleaved caspase-3 in SGC-7901 cells treated with 20 µM WA and 10 Gy IR at different time points (1, 3, 6, and 18 h). (F) Cleavage of poly ADP-ribose polymerase (PARP) in SGC-7901 cells following combined WA and IR treatment. Data are representative of three independent experiments.
