Fig. 2
The combination of WA and radiation induces autophagosome accumulation. (A-B) Western blot analysis of the autophagy marker protein LC3 in gastric cancer cells treated with 20 µM WA and 10 Gy radiation for 1 h, 3 h, 6 h, and 18 h (Raw blots are shown in Figure S1). Representative images from at least three independent biological replicates are presented. β-Actin was used as a loading control. (C-D) Confocal microscopy images showing EGFP-LC3 puncta (green), with cell nuclei stained using DAPI (blue). All images were acquired using identical confocal microscope settings (laser intensity, exposure time, and gain) across all groups to ensure comparability. All results are expressed as mean ± SD (n = 3). *P < 0.05, **P < 0.01, and ***P < 0.001.
