Table 3 Sequences of primers and antibodies for detected genes.
Gene | Sequence (5’ to 3’) |
|---|---|
Avpr1a -F | ATTCGCCAAGGATGACTCGG |
Avpr1a -R | TGGATATGCGGCTCAAGTGG |
Pdia2 -F | CGCTTCCTCGTCATACATAGCA |
Pdia2 -R | AGCAGGTGGTTGAGGATCTTG |
Nrtn -F | TCTGTCTGGATGTGCCAGGA |
Nrtn -R | GAAAGTTCTCGAAGCTCCACC |
Il-6 -F | TACCACTTCACAAGTCGGAGGC |
Il-6 -R | CTGCAAGTGCATCATCGTTGTTC |
mTnf-alpha -F | CCACCATCAAGGACTCAA |
mTnf-alpha -R | ATCTTATCCAGCCTCATTCT |
Il1b | QM03422S |
Nlrp3 | QM55990S |
Tgfb1 | QM05438S |
Tnf-antibody | ER65189, Huabio |
Nlrp3-antibody | ab263899 |
Safranine O and Fast Green Cartilage Staining | C0621S |
