Fig. 8
From: Antigenicity analysis of the recombinant outer membrane NlpI protein of Mannheimia haemolytica
IgG antibody generation against M. haemolytica strain 95 in reaction to rNlpI protein immunization (n = 10). A Immunization schedule for titer evaluation. B IgG titers against M. haemolytica strain 95, measured in the serum of BALB/c mice immunized with 30 µg rNlpI and 10, 20, and 30 µg rNlpI + white oil adjuvant; PBS and white oil adjuvant acted as controls. Measurements started seven days post injection. C IgG subtype titers (IgG1, IgG2a, IgG2b, and IgG3) against M. haemolytica strain 95 in immunized mice were measured after the third immunization. D IgG subtype detection over time inthe four mouse rNlpI protein immunization groups. Two-way ANOVA with Dunnett’s multiple comparison test was used to compare the groups. **** P < 0.0001. Images are created using GraphPad.Prism.9.5 (https://www.graphpad-prism.cn/) and combined using Adobe Illustrator 2020 (https://helpx.adobe.com/cn/download-install.html) software.
