Table 3 The primer sequences used in this study.
From: Antigenicity analysis of the recombinant outer membrane NlpI protein of Mannheimia haemolytica
Gene | Sequence | Annealing temperature | Size (bp) |
|---|---|---|---|
nlpI | CCGctcgagATGGTACAACTTTTTAAGTTGTTTCG | 62.7 | 924 |
CCCaagcttCTATTCTTTATCAACAACGGGAGTC | |||
nlpI qPCR | GGTCGTTAGCCCGTTATGATTTTGC | 60.0 | 99 |
CGCCTTCCAGCAGCAGATACAG | |||
16s Mh | CACCTGCCATAAGATGAGCCCAAG | 62.0 | 147 |
CCCACTGCTGCCTCCCGTAG |
