Fig. 1
From: Dysregulation of the low-level replication stress response in transformed cell lines
Primary and transformed cells differentially produce ROS in response to replication stress. (A) HU- or APH-induced ROS production was monitored using the DCFDA fluorescent probe and FACS analysis. The shift in the fluorescence peak reveals the induction of intracellular ROS production. (B) Typical peak-shaped dose–response curve of primary fibroblasts or epithelial cells following replication stress10. (C) Dose response of ROS induction in three different human cell lines after 48 h of exposure to HU. (D) Dose response of ROS induction in V79 hamster cells after 48 h of exposure to HU. (E) Impact of exposure to HU on the cell cycle distribution. The data from at least three independent experiments are presented as the mean (± SEM) level of ROS production normalized to that of the control. The non-parametric Kruskal–Wallis test was used.
