Fig. 2
From: Development and in vitro characterization of humanized antibodies for blocking human TRPM4 channel
Affinity ranking of humanized antibodies. (A) Sensor-grams showing affinity ranking of antibodies Ab1-Ab7 to biotin-conjugated antigen. The off-rates of antibodies were obtained from fitting the experimental data locally to 1:1 interaction model. Ab1: chimeric antibody. Ab2-7: humanized antibodies with back mutations to the FR region. NC: negative control. Sample concentration 400 nM. (B) Sensor-grams of the affinity measurement of biotin-conjugated antigen to antibodies Ab1, 2, 4, and 6. Sample concentration used are (in nM) 200, 100, 50, 25, 12.5, 6.25, 3.125. The colored lines represent the recorded binding response signals at different antigen concentrations, and the overlaid black lines represent the fitted curves.
