Table 1 Binding kinetics of antibody to antigen. NC: negative control; RU: response units; ka: association rate constant; kd: dissociation rate constant; KD: the equilibrium dissociation constants, kd = kd/ka; rmax: maximum analyte-ligand interaction response.
From: Development and in vitro characterization of humanized antibodies for blocking human TRPM4 channel
Ligand | Analyte | Chi² (RU²) | ka (1/Ms) | kd (1/s) | KD (M) | Rmax (RU) |
|---|---|---|---|---|---|---|
NC | peptide-biotin | NA | NA | NA | NA | NA |
Ab1 | peptide-biotin | 1.61E-02 | 3.25E + 05 | 1.50E-03 | 4.61E-09 | 24.3 |
Ab2 | peptide-biotin | 2.24E-02 | 2.49E + 05 | 1.66E-03 | 6.66E-09 | 28.2 |
Ab3 | peptide-biotin | 2.66E-01 | 3.27E + 05 | 3.21E-03 | 9.81E-09 | 25.4 |
Ab4 | peptide-biotin | 4.42E-02 | 2.52E + 05 | 1.77E-03 | 7.00E-09 | 26.9 |
Ab5 | peptide-biotin | 8.68E-02 | 2.17E + 05 | 2.17E-03 | 9.96E-09 | 24 |
Ab6 (M4H) | peptide-biotin | 5.72E-02 | 1.84E + 05 | 1.95E-03 | 1.06E-08 | 31.1 |
Ab7 | peptide-biotin | 9.44E-02 | 2.56E + 05 | 2.95E-03 | 1.15E-08 | 29.6 |
