Fig. 7

Enhancement of Dorsal Fin Cell Growth in Zebrafish by ZXGD Treatment (A) Transverse evaluations of Tg(fli1: EGFP) zebrafish embryos conducted 20 h post-fertilization (hpf) under a microscope revealed that ZXGD treatment significantly promotes the growth of dorsal fin vasculature. (B) The relative survival rates of zebrafish embryos subjected to ZXGD treatment (100 μM/L) demonstrated no cytotoxic effects (p < 0.05), indicating the safety of ZXGD at the tested concentration.(C) Following treatment with ZXGD (100 μM/L), a notable increase in the relative length of intersegmental vessels (ISVs) was observed, with statistical significance (p < 0.001), underscoring the efficacy of ZXGD in enhancing vascular development within the dorsal fin region.