Fig. 1
Comprehensive sRNA-seq analysis of MB tissue microRNA profiles. (a) Heatmap of dysregulated miRNAs (control group D (D): single paediatric autopsy brain without pathology, n = 1; MB group C (C1-C9): n = 9. (b) Volcano plot of miRNA expression revealed 328 dysregulated miRNAs: 57 downregulated and 271 upregulated. miR-206 and miR-383 showed significant downregulation in all MB samples (p < 0.04 and p < 0.01, respectively). (c) KEGG32 pathway analysis revealed that dysregulated miRNAs were primarily associated with axon guidance, natural killer cell mediated cytotoxicity, and pathways in cancer (red boxes). (d) Biological process analysis showed that most dysregulated miRNAs were involved in processes such as the regulation of transcription and intracellular signal transduction (red boxes). (e) Molecular function analysis of dysregulated miRNA showed that they took part in metal ion binding and nucleic acid binding (red boxes). For sRNA-seq analyses, p-values were adjusted using the Benjamini–Hochberg method to control the false discovery rate. miRNAs with an adjusted p-value < 0.05 were considered differentially expressed. A q-value < 0.005 and |log₂(fold change)|≥ 1 were used as thresholds to define significant differential expression, capturing miRNAs with meaningful and potentially biologically relevant changes, even below the more stringent fold change cutoff of > 2.
