Fig. 3
Comprehensive transcriptomics analysis of MB tissue profiles. (a) Heatmap of dysregulated genes (control group D (D); MB group C (C1-C9). (b) Volcano plot of gene expression revealed 1013 dysregulated genes: 415 downregulated and 598 upregulated. CORO1C and SV2B were identified as upregulated in the patient sample cohort. (c) Pathway enrichment analysis revealed that DEG were primarily associated with the regulation of the cell cycle, synaptic vesicle cycle, axon guidance, and miRNAs in cancer (red boxes). (d) The molecular function analysis showed that the vast majority of DEG were involved in processes such as ATP binding and nucleic acid binding (red boxes). (e) Biological process analysis showed that most dysregulated genes were involved cell division, cellular response to DNA damage, and mitotic cell cycle checkpoints (red boxes). Transcriptomic and sRNA-seq data were generated from the same patient cohort (n = 9). For transcriptomics analyses, p-values were adjusted using the Benjamini–Hochberg method to control the false discovery rate. Genes with an adjusted p-value < 0.05 were considered differentially expressed. A q-value < 0.005 and |log₂(fold change)|≥ 1 were used as thresholds to define significant differential expression, capturing genes with meaningful and potentially biologically relevant changes, even below the more stringent fold change cutoff of > 2.
