Fig. 4
RT-qPCR validations within MB and GB cell lines. (a) miR-206 was significantly downregulated in MB cells in comparison to normal human astrocytes (p < 0.0001). (b) miR-383 was significantly downregulated in MB cells in comparison to normal human astrocytes (p < 0.0096). (c) CORO1C was significantly upregulated in MB cells in comparison to normal human astrocytes (p < 0.0218). (d) SV2B was significantly upregulated in MB cells in comparison to normal human astrocytes (p < 0.0001). (e) miR-206 was significantly downregulated in U87MG and U251MG GB cells in comparison to normal human astrocytes (U87MG, p < 0.0033; U251MG, p < 0.0024). (f) miR-383 was significantly downregulated in GB cells in comparison to normal human astrocytes (U87MG, p < 0.0002; U251MG p < 0.0009). (g) CORO1C was significantly upregulated in GB cells in comparison to normal human astrocytes (U251MG, p < 0.0002; U87MG p < 0.0001). (h) SV2B was significantly upregulated in GB cells in comparison to normal human astrocytes (U251MG, p < 0.0001; U87MG p < 0.0001). Results are based on three independent experiments, n = 3. Statistical analyses were conducted using ordinary one-way ANOVA, followed by Dunnett’s multiple comparisons test. When only two groups were compared, unpaired t-tests were used to determine significant differences in expression. Error bars represent the standard deviation (SD) of the mean. ns non-significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
